A Secret Weapon For flow cytometers

A Secret Weapon For flow cytometers

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Info-enriched characterization of mobile populations. Morphological information from visuals can incorporate for the richness of flow cytometry facts. One example is, the determine shows an in any other case typical apoptosis assay working with Annexin V and PI, including cell imaging to characterize cells in Each individual population to expose morphologically unique attributes. These insights could not have been acquired from multiplex staining on your own.

Regardless of whether you’re a rookie or a specialist, check out our sources to advance your analysis and abilities in flow cytometry.

Merge acoustic concentrating technological know-how with substantial-parameter Investigation for adaptable spectral unmixing and conventional workflows.

Uncover new insights into your experimental details by visualizing populations making use of mixed fluorescence and morphology information. Our computer software interface wizards empower downsampling, concatenation (merged documents) and dimensionality applications that are crafted into your software program for an intuitive, streamlined facts analysis working experience.

"An image isn't really very truly worth a thousand dots, however it definitely helps you to see visuals. It is excellent to determine pictures that correlate with staining. One example is, AnnexinV-constructive cells which are morphologically diverse than nutritious cells, or CD14+ monocytes which have been much larger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.

In this article, a qualified user has gated singlets confidently. Right after analyzing the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate includes much more than four% aggregates.

Optimizing gating approach. Even robust guide singlet gating is mistake-prone and stays a subjective choice stage in Nearly all flow cytometry assays. Imaging can be used to confirm and regulate gates to include only single cells of desire.

Our researchers will design panels to suit your needs as repeatedly you require—free of charge, no products invest in needed.

The Attune NxT cytometer may be the trustworthy workhorse instrument your lab can depend upon for regular and dependable performance. The Attune CytPix model includes a substantial-speed brightfield digicam and AI-driven picture Investigation software program, with the latest updates like machine Studying resources and an intuitive person interface, enabling enhanced knowledge precision, precision, and more quickly insights into sample biology.

Maybe most significantly, these occasions consist of cells of Evidently unique phenotypes which could lead on to incorrect conclusions regarding double good events (specifically in exceptional populations).

Seem waves help precise mobile alignment, causing Extraordinary knowledge high-quality and ~10X more quickly sample flow rates.

We Beforehand shown the strength of imaging CAR-T/Ramos cell interactions. Let’s evaluate just the inhabitants of biggest desire, the double favourable occasions, to learn more. We will now use extended morphological parameters (below, circularity and skewness of intensity) to more study the attributes of such populations and refine gating on these gatherings, increasing knowledge robustness.

Leverage the strength of advanced technological know-how and innovation to speed up your discoveries. Have confidence in while in the Attune Flow Cytometer family members to provide the dependability, speed, and precision that set your function aside.

No clean/no lyse leukocyte Assessment with imaging. Cells have been obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Qualified UX/UI allows end users to educate AI models for expanded programs. (A) The “Prepare Design” element provides annotation equipment and actual-time design education precision.

At the heart of each and every check here Attune Flow Cytometer lies our revolutionary acoustic focusing technological know-how, which utilizes audio waves to meticulously align cells inside the flow cell. This ground breaking tactic surpasses traditional hydrodynamic approaches, resulting in more rapidly processing speeds, Remarkable information top quality, and elevated sample throughput.

As the mobile line experienced Beforehand been analyzed within the Attune CytPix Flow Cytometer, the researchers went back again to the images and have been in the position to doc the microbial infection at least five times earlier.

Agilent Price Assure assures you at the very least a decade of instrument use from your date you buy. If not, we will credit score you With all the residual worth of the procedure towards an upgraded model.

Below, we present that by gating procedures applying image-based mostly quantitative parameters can distinguish interacting cells from coincident occasions to more precisely analyze interacting cells.